High Performance Liquid Chromatography Column Structure:
Empty column assembled from the column joints, tube and frits ,end fittings .
In the two end fittings, a stainless steel filter (or strainer) is placed on both ends of the column for sealing the column packing and is not lost by the mobile phase. Empty column of the components are 316L stainless steel made can withstand the general solvent.because of the chloride-containing solvent to its corrosive, it should be noted that the column and the connection tube can not be long-term retention of such high chemical solvents to avoid corrosion.
The column installation
1)open the column box to confirm the type of column, size, date and the solvent stored in the column.
2) Remove the end fittings of the column at both ends of the sealing plug back into the box for backup.
3)Connect the inlet end of the column through the connecting pipe to the inlet of the injection valve (if the conditions permit, it is recommended to use the column before the column); the outlet of the column is connected with the detector. The connecting pipe is a stainless steel pipe ,be sure to try to reduce the dead volume outside the column when taking over. Connect the tube through the hollow screw, press the ring as far as possible after the force in the end, and then tighten the hollow screw clockwise until the screw does not move so far, remember not to force too much. If the column is pressurized by the mobile phase, use a wrench to continue 1/4 turn clockwise until it without leaks.
Use Tips of HPLC Columns:
Prior to use, the column is best tested for performance and the results are stored as a reference for future evaluation of column performance changes. Note that column performance may vary depending on the conditions used for the sample, mobile phase, column temperature, etc.
In addition, the column performance test is performed in accordance with the conditions in the column factory report (factory test The conditions are the best conditions), the only way, the measured results are comparable.
After production finished, all Starlab HPLC products will send to QC department to do quality inspection.This way ensure only qualified products can release to market. Besides, certificate of Analyse will be sent to clients with products together.
More product info with different sorbents,kindly visit our website for detail. http://www.starlabsci.com/