How to Store the Chromatography Column?

1.Prevent the generation of microorganisms from the buffer solution and the mobile phase of the aqueous solution, make sure that the mobile phase is used in a small amount, or add 200 ppm of sodium azide to the mobile phase of the water to inhibit bacterial growth (beware of its toxicity and potential explosiveness); or in the mobile phase Adding 20% of the organic modifier can also inhibit the growth of microorganisms. The organic modifier also helps to degas the mobile phase.

2.Store the column as much as possible in 100% organic solvent (Beiqing best) and avoid storing in buffer solution.

3.After the buffer solution was applied to the column, 15 to 20 column volumes of the same kind of water-organic solvent mobile phase containing no buffer were used to wash the column and then stored in a 100% organic solvent.

4.Avoid flushing tightly bonded C18 columns with purified water.

5.Twist the ends of the column with a plug to prevent the column bed packing from cracking.

Vacuum Filtration Manifolds

Starlab Scientific stocks a full variety of Vacuum Filtration Manifolds .

The material of Vacuum Filtration Manifolds are glass and stainless steel. There are three types of Vacuum Filtration Manifolds for glass series, one-way, three-way and six-way, as well for the stainless steel series. The integrative filter with reasonable structure and beautiful contour and without wedding spots, equipped with separate valve so that it can filter single sample or multiple samples at the same time so as to save time when testing a lot of samples.Starlab solvent filter made with 316L stainless steel has the characteristics of acid-resistance, alkali resistance, fast filtering speed, long service life, reasonable structure, easily cleaning and operating. It can be widely used in the fields of chemical industry, biochemical pharmacy, environmental protection, food and beverage etc.

All of Starlab Scientific’s Vacuum Filtration Manifolds are manufactured under ISO9001, and the strictly quality control system ensure variety of requirements from customers are met. Lots of good feedbacks from them encourage us to continue produce high-level pipettes.

Applications of Vacuum Pumps

Vacuum pumps are used in many industrial and scientific processes including composite plastic moulding processes, production of most types of electric lamps, vacuum tubes, and CRTs where the device is either left evacuated or re-filled with a specific gas or gas mixture, semiconductor processing, notably ion implantation, dry etch and PVD, ALD, PECVD and CVD deposition and so on in photolithography, electron microscopy, medical processes that require suction, uranium enrichment, medical applications such as radiotherapy, radiosurgery and radiopharmacy, analytical instrumentation to analyse gas, liquid, solid, surface and bio materials, mass spectrometers to create a high vacuum between the ion source and the detector, vacuum coating on glass, metal and plastics for decoration, for durability and for energy saving, such as low-emissivity glass, hard coating for engine components (as in Formula One), ophthalmic coating, milking machines and other equipment in dairy sheds, vacuum impregnation of porous products such as wood or electric motor windings, air conditioning service (removing all contaminants from the system before charging with refrigerant), trash compactor[citation needed], vacuum engineering, sewage systems (see EN1091:1997 standards), freeze drying, and fusion research. In the field of oil regeneration and rerefining, vacuum pumps create a low vacuum for oil dehydration and a high vacuum for oil purification.[12] Especially in the field of transformer maintenance, vacuum pumps play an essential role in transformer oil purification plants which are used to extend the lifetime of transformers in the field.[13]


Vacuum may be used to power, or provide assistance to mechanical devices. In hybrid and diesel engine motor vehicles, a pump fitted on the engine (usually on the camshaft) is used to produce vacuum. In petrol engines, instead, vacuum is typically obtained as a side-effect of the operation of the engine and the flow restriction created by the throttle plate, but may be also supplemented by an electrically operated vacuum pump to boost braking assistance or improve fuel consumption. This vacuum may then be used to power the following motor vehicle components:[14] vacuum servo booster for the hydraulic brakes, motors that move dampers in the ventilation system, throttle driver in the cruise control servomechanism, door locks or trunk releases.


In an aircraft, the vacuum source is often used to power gyroscopes in the various flight instruments. To prevent the complete loss of instrumentation in the event of an electrical failure, the instrument panel is deliberately designed with certain instruments powered by electricity and other instruments powered by the vacuum source.

Starlab Flash Column Using Guidance

Prepacking the Flash Column

  • Remove the end fittings ;
  •  Eliminates the thermal effect from solvent adsorption on a dry cartridge;
  • Ensures pre-equilibrium between the solvent and the silica .
  • Entrapped air ,the thermal effect and lack of equilibrium can cause separation, purity and recovery  problems ,especially with early eluting compounds .
  • In general the recommended pre-equilibration volume is 3-5 column volumes prior to sample loading. If using gradient elution ,pre-equilibrate with the starting solvent mixture.


Sample Loading

  • Liquid loading: Each column is supplied with a built-in inserter which allows liquid injection via a syringe.
  • Dry loading: If internal dry loading is to be used,this inserter must be removed.
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step 1  step 2  step 3  step 4
 图片5  图片6  图片7  图片8
 step 5  step 6  step 7  step 8

Influencing SPE Cartridge Factor

There are several factors influencing the extraction efficiency:

(1) Packing (fixed phase)

Selecting the appropriate SPE column filler is the prerequisite for the ideal result.

(2) The strength of the solvent

1. With positive phase fixation, the strength of solvent increases with its polarity

2. With the reverse stationary phase, the strength of solvent increases with the weakening of the machine

(3) PH value

Ion exchange fixed phase, analyte and interference matter have different PKa. By adjusting the PH, the stationary phase can be charged with opposite charge and the interfering material has no charge. Or, in turn, the stationary phase is charged with the opposite charge, while the analyte does not carry the charge, thus achieving the purpose of separation and purification

Starlab QuEChERS Extraction Salt

Starlab QuEChERS Extraction Salt Packed with pre-weighed anhydrous extract salt, where anhydrous magnesium sulfate is used to remove moisture from the sample matrix and other buffers are used to provide the appropriate pH to ensure that some alkali-sensitive pesticides can Good recovery rate.

When a large sample of water content is encountered, the buffered salt reacts exothermically with water, thereby affecting the recovery rate. Starlab provides a separate extraction of salt packets, the user can join the organic solvent and then add the buffer salt, to prevent local overheating of the sample.


  •  Convenient packing and no contamination;
  •  All components are pre-accurate weighing,effectively improve the labor efficiency of the laboratory;
  •  Fully automated production, to eliminate the error of artificial packaging to ensure the repeat-ability of the analysis results.
  •  Extracted salt by special optimization, no impurity interference can ensure the recovery and sensitivity of multi-residue analysis.
  •  Easy to tear bag packaging. Easy to transport and storage, and can greatly reduce the use of cost.


  •  Sample extraction liquid purification;
  •  Food, environmental water samples and soil contaminants
  •   Analysis and validation of multi-residual pesticides in Vegetables and Fruits.


EU EN method and AOAC both available.

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 图片3  图片4

Problem When Use HPLC

(1) Mobile phase: must be used after chromatography through the filter after use, and the mobile phase ratio can not be completely in accordance with the standard.


(2) Abnormal column pressure: the bubble inside the column, with the syringe to the bubble out, the column pressure back to normal. Column height: internal obstruction problems occur.


(3) Accuracy of external standard method: the accuracy of the external standard method related to the determination of the results, so the injection must be accurate results, the standard peak area RSD value should be less than 2.0. The general injector should not have bubbles present when extracting samples.


(4) When the test sample or standard peak pinning phenomenon, should check whether the phenomenon of collapse of the column occurred, if any, then re-column or change the new pillars.

HPLC chromatography Column Application ——-in the Detection of Drug

Life Science,health care and pharmaceutical were get more and more focuses by human beings as fast develop of scientific technique. In the drug analysis, high performance liquid chromatography is widely used in the quantitative analysis of drug analysis because of its specificity,.With feature of high sensitivity, fast and simple characteristics, especially in the interference factors show more superiority.

Determination of Lomefloxacin in Lomefloxacin Hydrochloride Eye Drops by HPLC Column; Column C, 0.02tool / nm Acid Solution (pH adjusted to 2.6 with triethylamine) – acetonitrile (R = 0.9998), the average recovery was 100.4, and the RSD0 of the repeated injection was in the range of 0.48 ~ 0.72 g, and the peak was 287 nm. .33 (n-6).

Zhang Min and other high-performance liquid chromatography with high-performance liquid chromatography determination of baicalin in addition to the domestic also on the quinolones antibiotics n, compound amoxicillin powder amoxicillin and clavulanic acid mouth, compound cold liquid, Qinlian Oral solution of baicalin, hydrobromide dextromethorphan tablets, feed chloropyridine, injection of neviomycin sulfate, small oxytetracycline C2b, health food in the sildenafil and celestium, plant more The injury of camptothecin and its derivatives, feed thirteen kinds of sulfonamides and other drugs were studied. In the use of high performance liquid chromatography for determination with other technologies, the detector is generally UV spectrophotometer, mass spectrometry and so on. These sophisticated detectors determine the sensitivity of the analysis, which is important for the analysis and is an important component of high performance liquid chromatography (HPLC).

Starlab HPLC column with ideal anti-pressure tube made from SS316 Stainless Steel can be wide chemical compatible for global users application.C18 Universal and C8 Universal especially equivalent to Waters brand,100 000 grade clean room made sorbents with perfect efficiency and 100% no contamination.

With development of pharmaceutical and R&D of chromatography,Starlab will keep on bring more columns to make more users satisfaction and do contribute to life science and pharmaceutical industries.


Brief Introductionof Normal Phase Chromatography

In the early eighties, the complex chromatographic stationary phase silica gel and pyridine thiocyanate nickel salt complex with the aromatic compounds to form inclusions for the separation of aromatic nitrogen isomers and cholesteric crystals. It has been the coke adsorbent as filler and bonded silica were compared and studied its thermodynamic mechanism. Large-pore polymers with ionized or non-ionizing functional groups also begin to be applied to liquid chromatography, which are stable over the entire acid-base range.


One of the disadvantages is that they will shrink and shrink when the solvent changes, but The main analytical aspects of the use, that is, the collection of traces of compounds from the water is no problem, through the appropriate treatment into the column, some substances appear excessive peak band widening, while others appear sharp, narrow peak And high resolution, in the strong alkaline porous resin 120 minutes can be separated from 100 kinds of urine in the UV absorption of substances. Some of the chain length of the carbon-hydrogen ligand bonded to the silica gel, according to the chain length and short effect of the retention value of the study, there have been similar reports, the C22 bond phase and the preparation of bonding phase load conditions In comparison, the bond length and the adsorption free energy can be linearly related. In general, the alkaline solution will destroy the silica gel, alkylamine than quaternary ammonium salt will corrode silicone filler, so by filling with 5mm silica gel short column to elute alkaline solution. Kataev et al with polytrifluorostyrene coated silica gel particle matrix and applied to the separation of halogenated aromatic hydrocarbons, peptides and proteins.


In November 1993, Majors discussed the rapid development of HPLC polymer fillers in Japan. Hosoya prepared the macroporous polymerization of ethylene-p-tert-butylbenzoate microspheres and two other polymer microspheres with more properties as well as more C18HPLC stationary phases.

HPLC Column Installation and Using Tips

High Performance Liquid Chromatography Column Structure:

Empty column assembled from the column joints, tube and frits ,end fittings .

In the two end fittings, a stainless steel filter (or strainer) is placed on both ends of the column for sealing the column packing and is not lost by the mobile phase. Empty column of the components are 316L stainless steel made can withstand the general solvent.because of the chloride-containing solvent to its corrosive, it should be noted that the column and the connection tube can not be long-term retention of such high chemical solvents to avoid corrosion.


The column installation

1)open the column box to confirm the type of column, size, date and the solvent stored in the column.

2) Remove the end fittings of the column at both ends of the sealing plug back into the box for backup.

3)Connect the inlet end of the column through the connecting pipe to the inlet of the injection valve (if the conditions permit, it is recommended to use the column before the column); the outlet of the column is connected with the detector. The connecting pipe is a stainless steel pipe ,be sure to try to reduce the dead volume outside the column when taking over. Connect the tube through the hollow screw, press the ring as far as possible after the force in the end, and then tighten the hollow screw clockwise until the screw does not move so far, remember not to force too much. If the column is pressurized by the mobile phase, use a wrench to continue 1/4 turn clockwise until it without leaks.


Use Tips of HPLC Columns:

Prior to use, the column is best tested for performance and the results are stored as a reference for future evaluation of column performance changes. Note that column performance may vary depending on the conditions used for the sample, mobile phase, column temperature, etc.

In addition, the column performance test is performed in accordance with the conditions in the column factory report (factory test The conditions are the best conditions), the only way, the measured results are comparable.


After production finished, all Starlab HPLC products will send to QC department to do quality inspection.This way ensure only qualified products can release to market. Besides, certificate of Analyse will be sent to clients with products together.


More product info with different sorbents,kindly visit our website for detail.